Cell Migration under confinement Workshop 2018
ReTuBi Lecture - Kristine Schauer
On April 26 of 2018, Kristine Schauer from Institut Curie (France) was the invited speaker as an expert visit with the lecture “Role of actin - and microtubule - dependent motor proteins in Golgi positioning and trafficking”.
Summary of the Lecture:
Many cellular disorders are accompanied by changes in the morphology and positioning of intracellular organelles, yet the functional consequences of organelle alterations are often not clear. The Golgi apparatus is a large and distinctive organelle in mammalian cells constituting the biggest hub of intracellular trafficking at the interface between anterograde and retrograde routes. Although the Golgi apparatus was one of the first organelles to be discovered and observed in detail, the function of its compact structure remains enigmatic. We study cells on bioengineered surfaces of micro-fabricated patterns that provide adhesive cues for defined cell spreading. This approach reduces cell-to-cell variation and mimics space restriction which cells encounter in tissues. We combine this controlled cell culture condition with precise quantification of organelle positioning using a density-based imaging approach. We have previously shown that the Golgi apparatus has a characteristic, well-defined, stable and reproducible organization in micropatterned cells. Because morphology and spatial positioning of organelles are regulated by the cytoskeleton and molecular motor proteins, we are interested in the role of motors at the Golgi. We have used our density-based approach on micropatterns to screen for actin-dependent myosins and microtubule-based kinesins that alter the morphology of the Golgi apparatus. We have identified Myo1C and Kif5B as critical players and have further characterized their role in Golgi positioning, trafficking and cell function. Our analysis will be helpful to understanding the molecular mechanisms underlying intracellular changes associated with cellular pathogenesis such as cancer.
Staff exchange between iMM-Curie (2018/04)
From April 10 to 17 of 2018 Catarina Fonseca from Cláudio Franco (iMM) made a “ReTuBi Staff Exchange” to Franck Perez (Institut Curie) to increase the experience of Catarina Fonseca PhD project which aims to perform a high throughput screening to silence different proteins and observe how is the Golgi position under different conditions using live imaging. To do that, it is necessary to use not only live-imaging techniques as well as use a high throughput analysis that will help to obtain the results. Since FrancK Perez Lab works in Golgi dynamics and already use and establish the high content screening, Catarina Fonseca was able to to get knowledge in this specific technique.
From April 10 to 12 of 2018 Maria Cejudo and Isabela Fortunato from Cláudio Franco (iMM) together with Cláudio Franco made a “ReTuBi Staff Exchange” to Danijela Vignjevic Lab (Institut Curie) to discuss ongoing collaborative projects on gut microvasculature and NM-MIIA function in endothelial cells.
From April 10 to 19 of 2018 João Martins from Edgar Gomes Lab (iMM) made a “ReTuBi Staff Exchange” to Danijela Vignjevic Lab (Institut Curie). The main goal of this staff exchange was to share knowledge about techniques that are required for the development of the projects in each lab. With this, there also the added bonus of being able to interact with many international researchers, improving João Martins communication skills and networking. Danijela Vignjevic’s laboratory has extensive expertise in extracellular matrix alterations by matrix proteinases and co-cultures, which is something that João Martins need to optimize for his PhD project.
From April 10 to 19 of 2018 Raquel Duarte from Luís Costa Lab (iMM) made a “ReTuBi Staff Exchange” to Danijela Vignjevic Lab (Institut Curie). The research project of Raquel Duarte is focus in colorectal cancer. Danijela Vignjevic laboratory at Institut Curie focus on cell migration and invasion, using gut as a model. With this staff exchange, Raquel Duarte was able to learn the techniques used in Danijela Vignjevic laboratory, namely the production of 2D and 3D in vitro cell cultures, that could be then implemented in Luís Costa lab to complement their studies. Is also a great opportunity to discuss results, expand team network and to establish possible collaborations.
From April 10 to 19 of 2018 Francisca Vasconcelos from Cláudio Franco (iMM) made a “ReTuBi Staff Exchange” to Franck Perez (Institut Curie). The Franck Perez Lab studies vesicle intracellular trafficking and has previously establish a molecular system for controlled secretion of cargo proteins (RUSH system) and a high-throughput screening for automated analysis. The staff exchange objective was to define the best strategies to implement a high-throughput screening of drugs that control VEGF secretion using the RUSH system. Thus, Francisca Vasconcelos was able to acquire new technical skills that are important for the development of new research project at Cláudio Franco Lab.
Joint Lab Retreat on Cancer Biology
From April 04 to 06 in Douro (Portugal) it was held the Joint Lab Retreat on Cancer Biology between João Barata Lab (iMM) and François Radvanyi (Institute Curie) with a total of 37 participants.
Staff exchange between iMM-Curie (2018/03)
From from March 24 to 31 of 2018, Edgar Gomes (iMM) made a "ReTuBi Staff Exchange" to Institut Curie to meet with multiple group leaders and discuss current and future collaborations. Edgar Gomes met with Matthieu Piel to follow up on the staff exchange of his student Catia Janota, that was at Institut Curie from the January 20 to March 4 of 2018, working on cell confinement during cell migration.
Edgar Gomes also met with Renata Basto to follow up on previous contacts regarding how polyploid cells repair DNA upon DNA damage. A meeting with Phillipe Chavrier was also scheduled to discuss the current collaboration regarding cell migration of breast cancer cells through collagen matrices and to understand the role of nuclear squeezing in this process. Edgar Gomes also met with Danijela Vignjevic to discuss the role of myofibroblasts in tumour invasion and tissue morphogenesis. A meeting with Cecile Sykes was planned to discuss a current project, financed by the ANR-France, to study nucleo-cytoskeleton connections.
Finally, during this staff exchange, informal meetings with students and post-docs from these labs were also proposed.
ReTuBi Lecture - Jean-Léon Maître
On March 22 of 2018, Jean-Léon Maître from Institut Curie (France) was the invited speaker as an expert visit with the lecture “Mechanics of blastocyst morphogenesis”.
Summary of the Lecture:
During pre-implantation development, the mammalian embryo forms the blastocyst, which will implant into the uterus. The architecture of the blastocyst is essential to the specification of the first mammalian lineages and to the implantation of the embryo. Consisting of an epithelium enveloping a fluid-filled cavity and the inner cell mass, the blastocyst is sculpted by a succession of morphogenetic events. These deformations result from the changes in the forces and mechanical properties of the tissue composing the embryo. Using microaspiration, live-imaging, genetics and theoretical modelling, we study the biophysical and cellular changes leading to the formation of the blastocyst. In particular, we uncover the crucial role of acto-myosin contractility, which generates periodic waves of contractions, compacts the embryo, controls the position of cells within the embryo and influences fate specification.