Staff exchange between iMM-Curie (2017/07)
From July 5-9 of 2017, Simão Teixeira da Rocha from Maria do Carmo Fonseca Lab (iMM) made a “ReTuBi Staff Exchange” to Edith Heard Lab (Institut Curie) to finalize a manuscript in collaboration in order to submit it in a high impact journal.
From July 7-23 of 2017, Francisca Vasconcelos from Cláudio Franco Lab (iMM) made a “ReTuBi Staff Exchange” to Franck Perez Lab (Institut Curie) to improve new technical skills on vesicle intracellular trafficking and molecular system for controlled secretion of cargo proteins and a high-throughput screening for automated analysis.
ReTuBi Lecture - João Matos
On July 3 of 2017, João Matos, from ETH Zürich (Switzerland) was the invited speaker as an expert visit for the lecture “Transcriptional and metabolic regulation of vascular form and function”.
Summary of the lecture:
To prevent the catastrophic segregation of incompletely replicated chromosomes, eukaryotic cells target stalled replication intermediates for nucleolytic resolution in mitosis. The MUS81 endonuclease plays a pivotal role in this process, but whether and how cells prevent it from cleaving related DNA structures arising during unperturbed S-phase was unclear. In recent work we showed that MUS81 is active throughout the cell cycle, but requires association to the SLX4 scaffold for efficient substrate targeting. To preclude toxic processing of vital replication intermediates, WEE1 kinase restrains CDK1-mediated MUS81-SLX4 assembly during S-phase. Accordingly, WEE1 prevents widespread recruitment of MUS81-SLX4 to S-phase chromosomes and WEE1 inhibition elicits extensive cleavage of replication intermediates, triggering chromosome pulverization. Our work suggests that temporally restricted MUS81-SLX4 association suppresses toxic breakage of replication intermediates, while promoting resolution of under-replicated DNAs that would otherwise impair chromosome segregation.
ReTuBi Lecture - András Simon
On June 26 of 2017, András Simon, from Karolinska Institute, Department of Cell and Molecular Biology (Sweden) was the invited speaker as an expert visit for the "ReTuBi Lecture" with the talk “Salamander regeneration - regulation and evolution”.
Summary of the lecture:
The ability to regenerate lost body structures is present in diverse animal species ranging from simple organisms, such as the hydra to complex vertebrates, such as salamanders. We aim to understand how animals with outstanding regenerative capabilities sense what and how much is missing in relation to the normal homeostatic state, and how they translate that information to the appropriate regenerative responses. We primarily study an aquatic salamander, the newt, which possesses exceptional regenerative capacities among adult vertebrates. In particular, we focus on how progenitor cells are created and characterize their developmental potential during regeneration. We are currently generating comprehensive genetic resources such as transcriptome, genome databases, and in vivo genome editing tools for newts. We will use these genomic resources for gaining deeper insights into salamander regeneration at the molecular level and for cross-species comparisons with mammals in clinically relevant lesion/regeneration settings. Our research might reveal fundamental aspects of cell fate determination that could contribute to the design of novel regenerative strategies.
ReTuBi Lecture - Elodie Segura
On June 21 of 2017, Elodie Segura, from Institut Curie (France) was the invited speaker as an expert visit for the "ReTuBi Lecture" with the talk “Functional specialization of human dendritic cell subsets”.
Summary of the lecture:
Dendritic cells (DC) initiate and orient immune responses in lymphoid organs. DC are a heterogeneous population composed of several subsets with distinct phenotype and ontogeny. Mouse DC subsets have been shown to have distinct functions, especially in terms of antigen presentation and T cell stimulation. Whether the same is true for human DC subsets remains unclear. We have analyzed human DC directly purified from lymphoid organs and tissues, and found that some functional specializations are conserved between mouse and human DC, but others are not. These results have important implications for the rationale design of DC-based therapies.
Staff exchange between iMM-Curie (2017/06)
From June 18-23 of 2017, Inês Gomes, from Luís Costa Lab (iMM) made "ReTuBi Staff Exchange" to Marina Glukhova Lab (Institut Curie) to get new expertise in protocols for the isolation of mammary epithelial cells and the mammosphere assay serving to study the clonogenic potential of various mammary cell populations.
ReTuBi Lecture - Katie Bentley
On June 6 of 2017, Katie Bentley, from the Harvard Medical School (USA) was the invited speaker as an expert visit for the "ReTuBi Lecture" with the talk “Modelling endothelial cell behaviour in pathological angiogenesis”.